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Genesis. 2010 Mar;48(3):183-94. doi: 10.1002/dvg.20604.

Dynamic expression of a LEF-EGFP Wnt reporter in mouse development and cancer.

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  • 1Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, USA.

Abstract

We have characterized a transgenic mouse line in which enhanced green fluorescent protein (EGFP) is expressed under the control of multimerized LEF-1 responsive elements. In embryos, EGFP was detected in known sites of Wnt activation, including the primitive streak, mesoderm, neural tube, somites, heart, limb buds, mammary placodes, and whisker follicles. In vitro cultured transgenic embryonic fibroblasts upregulated EGFP expression in response to activation of Wnt signaling by GSK3beta inhibition. Mammary tumor cell lines derived from female LEF-EGFP transgenic mice treated with the carcinogen 7, 12-dimethylbenz[a]anthracene (DMBA) also express EGFP. Thus, this transgenic line is useful for ex vivo and in vitro studies of Wnt signaling in development and cancer.

PMID:
20146356
PMCID:
PMC3399184
DOI:
10.1002/dvg.20604
[PubMed - indexed for MEDLINE]
Free PMC Article
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