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Am J Pathol. 2018 Jun;188(6):1430-1446. doi: 10.1016/j.ajpath.2018.02.010. Epub 2018 Mar 12.

Induction of the Hajdu-Cheney Syndrome Mutation in CD19 B Cells in Mice Alters B-Cell Allocation but Not Skeletal Homeostasis.

Author information

  • 1Department of Orthopaedic Surgery, UConn Health, Farmington, Connecticut; UConn Musculoskeletal Institute, UConn Health, Farmington, Connecticut.
  • 2Department of Orthopaedic Surgery, UConn Health, Farmington, Connecticut; UConn Musculoskeletal Institute, UConn Health, Farmington, Connecticut; Department of Medicine, UConn Health, Farmington, Connecticut.
  • 3UConn Musculoskeletal Institute, UConn Health, Farmington, Connecticut.
  • 4Genome Engineering Technologies, Regeneron Pharmaceuticals, Tarrytown, New York.
  • 5Department of Orthopaedic Surgery, UConn Health, Farmington, Connecticut; UConn Musculoskeletal Institute, UConn Health, Farmington, Connecticut. Electronic address: asanjay@uchc.edu.
  • 6Department of Orthopaedic Surgery, UConn Health, Farmington, Connecticut; UConn Musculoskeletal Institute, UConn Health, Farmington, Connecticut; Department of Medicine, UConn Health, Farmington, Connecticut. Electronic address: canalis@uchc.edu.

Abstract

Mice harboring Notch2 mutations replicating Hajdu-Cheney syndrome (Notch2tm1.1ECan) have osteopenia and exhibit an increase in splenic marginal zone B cells with a decrease in follicular B cells. Whether the altered B-cell allocation is responsible for the osteopenia of Notch2tm1.1ECan mutants is unknown. To determine the effect of NOTCH2 activation in B cells on splenic B-cell allocation and skeletal phenotype, a conditional-by-inversion (COIN) Hajdu-Cheney syndrome allele of Notch2 (Notch2[ΔPEST]COIN) was used. Cre recombination generates a permanent Notch2ΔPEST allele expressing a transcript for which sequences coding for the proline, glutamic acid, serine, and threonine-rich (PEST) domain are replaced by a stop codon. CD19-Cre drivers were backcrossed into Notch2[ΔPEST]COIN/[ΔPEST]COIN to generate CD19-specific Notch2ΔPEST/ΔPEST mutants and control Notch2[ΔPEST]COIN/[ΔPEST]COIN littermates. There was an increase in marginal zone B cells and a decrease in follicular B cells in the spleen of CD19Cre/WT;Notch2ΔPEST/ΔPEST mice, recapitulating the splenic phenotype of Notch2tm1.1ECan mice. The effect was reproduced when the NOTCH1 intracellular domain was induced in CD19-expressing cells (CD19Cre/WT;RosaNotch1/WT mice). However, neither CD19Cre/WT;Notch2ΔPEST/ΔPEST nor CD19Cre/WT;RosaNotch1/WT mice had a skeletal phenotype. Moreover, splenectomies in Notch2tm1.1ECan mice did not reverse their osteopenic phenotype. In conclusion, Notch2 activation in CD19-expressing cells determines B-cell allocation in the spleen but has no skeletal consequences.

PMID:
29545197
PMCID:
PMC5971225
[Available on 2019-06-01]
DOI:
10.1016/j.ajpath.2018.02.010
[PubMed - in process]
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